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About Myself
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100 µM RBD-Cys was incubated with 10x excess of TCEP in PBS for 30 min at room temperature and subsequently purified into PBS with 10 mM EDTA using Zeba spin columns (7 kDa), incubation for ~6 h at room temperature allowed for the reoxidation of disulfides prior to the addition of the azide linker as monitored by Ellman’s assay.Boucau Find A Prostitute
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