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After that chaos, I’m starving. So, I hit up the local market on the Kerkweg. Fresh produce everywhere! I grab some sausages (obviously) and some apples. But then, I see this guy selling cheese. I’m a sucker for cheese. I’m sampling everything, and before I know it, I’ve got a whole cheese platter in my hands. Oops!
Donate to Perry Park Memorial Bench for Craig Van Doorn Sr., organized by Steven Ostrowski
Pelleted at 1200 rpm for 5 min at 4°C and resuspended in 300 μl PBS before they were filtered over a 70 μm size strainer to obtain a single cell suspension ready for FACS analysis and sorting, cell size, granularity, and fluorescence intensity were analyzed per brain region (n = 3) with a MoFlo fluorescent activated cell sorter (Beckman Coulter, Mijdrecht, Netherlands). Approximately 2x104 events were counted and subsequently analyzed using Summit software version 4.3 (DAKO) to determine gates before cells were sorted. Microglia were defined as the percentage of all living, Sytox green negative, cells that showed CD11bpos/CD45low expression (Sedgwick et al., 1991).Doorn Erotic Massage
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