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So, I’m rollin’ outta bed, right? The sun’s peeking through my window, and I’m like, “Yoshii, let’s do this!” I grab my sketchbook, ready to hit the streets. Yoshii’s got this vibe, ya know? It’s a small city, but it’s packed with character. The streets are narrow, and the buildings are all crammed together like they’re having a party.
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We used a Tsc1fl/fl mouse which has exon 17 and 18 flanked with Lox sequences and electroporated a DNA construct encoding Cre-GFP into wild-type (WT) or mutant fetuses at embryonic day 15.5 (E 15.5). We performed Immunohistochemistry in both WT and mutant brains at P28 and confirmed that Cre-GFP expressing neurons in the mutant showed suppression of the TSC1 protein (Figures 1A,B) and increased phosphorylation of ribosomal protein S6 (p-S6) (Figures 1C,D).Yoshii Whore
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