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These results suggest that a simple difference in the mtDNA copy number is not critical for post-implantation development. For mtDNA mutation analysis, we selectively amplified mtDNA from all 88 samples derived from 27 embryos using polymerase chain reaction (PCR) and performed mtDNA sequencing via next-generation sequencing. The success rate for data acquisition of each sample were 92.9% (52/56) for pre-culture samples, and 96.9% (31/32) for post-culture samples. Supplementary Table S1 lists mutations for each sample with a heteroplasmy level of ≥90%.Ijuin Sexual Massage
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