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After that, I needed a pick-me-up. So I hit up this little café on Shōwa-dōri. They got the best matcha latte. I order one, and the barista’s like, “Sorry, we’re outta matcha.” Seriously? What’s with today? I’m about to lose it. But then, this cute dog walks in. I mean, like, the fluffiest golden retriever ever. Instant mood booster! I’m petting the dog, and I forget all my troubles. Dogs really are the best therapy, right?
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Plates were then washed with 1x PBS + 0.05% Tween 20 and blocked with 3% BSA PBS-Tween 20 for 2 h at 37 °C, plates were washed and serum was serially diluted in 1% BSA PBS-Tween 20 and incubated for 2 h at 37 °C.Ino Whore
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