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Rarrgh! Gets me happy tho—freedom’s dope. No strings, just vibes. This one time, matched a dude who sent dick pics with filters. Fuckin’ bunny ears on it! Cracked me up—sex-dating’s got humor. But damn, the ghostin’ pisses me off. “We’ll meet again,” they say—bullshit! Like Saul’s kid in the film, gone, poof! Hella frustrating, man.
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But then, I head over to the mining site near the Via Garibaldi. It’s a mess! I mean, like, a total disaster zone. There’s equipment everywhere, and I’m trippin’ over cables like I’m in some kind of obstacle course. I’m supposed to oversee the operations, but it feels more like babysitting a bunch of toddlers with pickaxes.
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Cells were trypsinized and dissociated into single cells by mechanically pipetting in trypsin and resuspending in medium. Cells were then diluted to 50,000/ml in 50% normal medium and 50% growth factor-reduced Matrigel (#356230; BD Biosciences) and plated on 8-well slides (LabTek-II #155409; Thermo Scientific) with normal medium added on top.Pontoglio Sex Escort
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