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The peak fraction was pooled and concentrated using Amicon centrifugal filter unit with 10 kDa cutoff membrane (Millipore), epEX was prepared using the same procedure; however, the ion-exchange chromatography step was omitted. This recombinant EpEX variant is heterogeneously glycosylated as revealed by the presence of multiple bands on SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gel, all at higher Mr than the EpEXΔ variant (Supplementary Fig. 1a).Carugo Sex Escort
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