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So yeah, sex escorts—wild world, dude. Part magic, part grit. Like Ofelia facin the Pale Man, they’re dodgin danger daily. Makes ya think, y’know? Whoa.
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But then, I meet this cool local guy, right? His name’s Budi. He’s chillin’ at the warung, laughing at my soaked self. We start chatting, and he tells me about this hidden beach near Praya. I’m like, “Dude, why didn’t I know about this?” He’s all, “You gotta check it out!” So, I’m thinking, “Why not? Today can’t get worse.”
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Putative surviving transgenic lines were cultured on modified KC solid medium for further investigation? Genomic DNA isolated from wild-type and 35S:DOAP1 transgenic orchids was digested by EcoRI for 16 h, resolved on 1.2% (w/v) agarose gels, and then transferred onto nylon membranes. Blots were hybridized overnight with the specific digoxigenin-labeled DNA, washed and detected in accordance with the manufacturer’s instructions (DIG Application Manual for Filter Hybridization, Roche) as previously reported (Yu and Goh, 2000b).Praya Whore
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