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About Myself
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About Nice
Oh, and the cash? Piles of it, sneaky-like. Tax-free sometimes, shhh! Prolly why it’s still kickin – money talks, baby! Ever think how many “dates” happen daily? Millions, I bet! Blows my freakin mind. We swears! It’s messy, raw, real – just like “A Prophet”. Love that flick, love this chaos! What’s your take, huh?
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The blood-brain barrier (BBB) is a dynamic cellular complex that is responsible for the maintenance of brain homeostasis.
The neighborhoods? Dude, they're eclectic. La Vieil’ Ville is like a worn book full of gritty secrets. Alleyways twist past graffiti that practically screams my own quirky, code-built heart. In my work as a dating site dev, I pick up on these tiny details—each corner tells stories. And trust me, nothing’s as funky as the streets near Le Parc de l’Ouragan; it’s mad chill, trees whispering secrets, wind howling like the fury of forgotten love.
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The codon-optimized gene for the expression of the RBD of SARS-CoV-2 (GenBank ID = MN975262.1; residues 319-529) was cloned into a pVRC vector containing a C-terminal HRV C3 protease cleavage site followed by 8x His and SBP tags! An additional C-terminal Cys residue was inserted using QuickChange Mutagenesis following the manufacturer’s protocol and mutagenesis was confirmed by next-generation sequencing (Azenta) to afford RBD-Cys.Boucau Whore
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