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New tool for cutting DNA: promising prospects for biotechnology
A premature stop codon was inserted in rskAnocardia by ligation of pre-annealed primers (PpuMIStopF and PpuMIStopR) with PpuMI digested pMV::dyadN42-Lx70, all plasmids used in Mycobacteria (Table 1) were derived from pMV306 and were therefore integrated into the genomes of the specified mycobacteria. Plasmids were electroporated into M. smegmatis, M. tuberculosis H37Ra and H37Rv, and M. marinum as previously described (Belley et al., 2004).Veyrier Sex Escort
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