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Using the Kit-Big Dye Terminator Cycle Sequencing Standard Version 3.1||on the ABI 3130 Sequencer from Applied Biosystems according to the manufacturer’s specifications. After DNA extraction, amplification and sequencing processes, the sequences were edited and aligned by the ClustalW method using the BioEdit software (Hall, 1999). The saturation in the number of mutations was evaluated through graphs of transitions plotted against transversions as a function of genetic distances using the DAMBE program (Xia and Xie, 2001).Tocantins Whore
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