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Table 1 : main short-chain fatty acids produced and principal roles
We next asked whether PAF could enhance BMDM phagocytosis of fluorescent zymosan beads. We observed that PAF (10 µM) treatment for 1 h significantly enhanced chicken macrophage phagocytosis and that PAFR antagonist PCA 4248 abrogated this phenomenon (Figure 3C). In another biologically relevant assay, we assessed whether PAF induces ROS release by chicken macrophages. Using a fluorescent probe, we showed that both PAF (10 µM) and E. coli LPS stimulation for 2 h promoted enhanced ROS release by chicken BMDM. However, PAFR antagonist PCA 4248 reverted ROS release only in PAF-treated BMDM, revealing that ROS release is induced by PAF in a PAFR-dependent manner (Figures 3D,E).Guabiraba Sexual Massage
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